Techniques for Sperm Immobilization Test

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An immobilization test for amoebiasis.

The demonstration of antibodies in syphilitic sera that will immobilize Treponema pallidum (Nelson and Mayer, 1949) led Cole and Kent (1953) to investigate and demonstrate that immune rabbit sera were capable of immobilizing Entamoeba histolytica, and to suggest that sera from human cases of amoebiasis might show the same phenomenon. Their rabbit sera did not show immobilizing activity to a hig...

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Triple touch sperm immobilization vs. single touch sperm immobilization in ICSI - a randomised trial

BACKGROUND Although different techniques for sperm immobilization have been described, their value has not been assessed in an adequately powered randomized study. The aim of this study was to compare two types of sperm immobilization methods prior to ICSI and to test the hypothesis that triple touch immobilization (TTIm) would lead to a higher (5% -65% up to 70%) fertilization rate (FR) than s...

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Mechanism of Sperm Immobilization by Escherichia coli

AIM To explore the influence of Escherichia coli on the motility of human spermatozoa and its possible mechanism. METHODS Highly motile preparations of spermatozoa from normozoospermic patients were coincubated with Escherichia coli for 4 hours. At 1, 2 and 4 hours of incubation, sperm motility was determined. The factor responsible for sperm immobilization without agglutination was isolated ...

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A digital method of sperm immobilization test: comparison to the conventional method.

Antisperm antibodies have been found in infertile patients and those causing immobilization of sperm are considered to be closely related to unexplained infertility. These antibodies are usually identified by a sperm immobilization test which involves counting motile sperm under microscope. This test is subjective as it relies on the judgement of the examiner with respect to sperm motility. In ...

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Characterization of Sperm Immobilization Factor from Escherichia coli and Its Receptor to Study the Underlying Mechanism of Sperm Immobilization

Sperm immobilization factor (SIF) was extracted and purified from Escherichia coli filtrate. Characterization of SIF using Liquid chromatography –mass spectrometry (LC-MS) revealed its sequence similarity to chaperone protein HchA of E. coli O157:H7. Further the effect of SIF on sperm functions such as acrosome reaction was studied and the results showed that SIF (500μg/ml) significantly inhibi...

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ژورنال

عنوان ژورنال: Archives of Andrology

سال: 1989

ISSN: 0148-5016

DOI: 10.3109/01485018908986841